Our CRISPR-Q Custom PCR Assays let you characterize CRISPR editing events in cultured human, mouse or rat cells. Just enter your chromosome number, cut site and guide RNA sequence into our custom design tool, and our advanced design algorithm will do the rest. It checks for the presence of gRNA sequence in the PCR product to ensure amplification of the correct region of interest. It also checks for off-target amplification to ensure that only the specific target is amplified and to provide an optimal user experience.
The simple and fast workflow enables the characterization of CRISPR-based gene editing events for a variety of applications, including gene knock-out or insertion functional studies, base editing and genome screening (CRISPRi libraries, reversible knockdowns).
For PCR-based characterization and confirmation of CRISPR editing events in human, mouse, or rat cells
For sequencing-based characterization and confirmation of CRISPR editing events in human, mouse, or rat cells
For direct enhanced PCR amplification to confirm CRISPR editing events in human, mouse, or rat cells