Assay Nameβ
dPCR Mutation Assay PIK3CA 86044 Human
GeneGlobe Cat No (Assay ID)β
DMH0000216
Speciesβ
Human (Homo sapiens)
Gene Symbolβ
PIK3CA
Gene aliasesβ
PI3K
Ensembl Gene IDβ
ENSG00000121879
Entrez Gene IDβ
5290
Genomic Mutation ID (COSV by COSMIC)β
COSV55881636
Legacy Mutation ID (COSM by COSMIC)β
COSM86044
Amino Acid Changeβ
p.E365K
Nucleotide Changeβ
c.1093G>A
Wildtype Alleleβ
G
Mutant Alleleβ
A
Mutation Strandβ
+
Mutant descriptionβ
Substitution - Missense
Positive Control Sequenceβ
TATATTTGAGTCTATCGAGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACCTACTTTTTTCTTTTAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAACACTCAAAGAGTACCTTGTTCCAATCCCAGGTAAGGAAGT
Amplicon lengthβ
154
Recommended Restriction Enzymeβ
AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validatedβ
dPCR wet-lab validated
Probe Fluorophoreβ
FAM/HEX or ATTO550/ROX
Quencherβ
Iowa Black
Primer Purificationβ
Desalted
Probe Purificationβ
HPLC