GeneGlobe ID: DMH0000274 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay EGFR 18427 Human

AA Mutation: p.E746_P753delinsVS
Nucleotide Mutation: c.2237_2257delinsTCT
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay EGFR 18427 Human
GeneGlobe Cat No (Assay ID)​
DMH0000274
Species​
Human (Homo sapiens)
Gene Symbol​
EGFR
Gene aliases​
ERBB1;ERRP;ERBB
Ensembl Gene ID​
ENSG00000146648
Entrez Gene ID​
1956
Genomic Mutation ID (COSV by COSMIC)​
COSV51781339
Legacy Mutation ID (COSM by COSMIC)​
COSM18427
Amino Acid Change​
p.E746_P753delinsVS
Nucleotide Change​
c.2237_2257delinsTCT
Wildtype Allele​
GAATTAAGAGAAGCAACATCTC
Mutant Allele​
GTCT
Mutation Strand​
+
Mutant description​
Substitution - Missense
Positive Control Sequence​
CATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTG
Amplicon length​
107
Recommended Restriction Enzyme​
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
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