dPCR LNA Mutation Assays

For detecting specific DNA sequence mutations related to cancer and oncogenesis

Duplex assay design detects mutated and wild-type sequences
Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
LNA-enhanced primers and probes increase assay specificity and sensitivity
Wet-lab tested dPCR assays available for more than 200 targets
Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
Intended for use with QIAcuity Probe PCR Kits

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Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.