GeneGlobe ID: DCG0000388 | Cat. No.: 250210 | dPCR CNV Probe Assays

dPCR CNV Probe Gene of Interest for USP9Y

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Product Specification

Gene symbol: USP9Y
Ensembl Gene ID: ENSG00000114374
dPCR wet-lab validated
Product Name​
dPCR CNV Probe Gene of Interest for USP9Y
GeneGlobe Cat.No. (Assay ID)​
DCG0000388
Assay type​
Gene of Interest
Species​
Human (Homo sapiens)
Gene symbol​
USP9Y
Ensembl Gene ID​
ENSG00000114374
NCBI Gene Id​
8287
Strand​
Binds to reverse (3’ to 5’) bottom genome strand
Amplicon length​
80
Amplicon region​
GTATCATATTATACTCATCGGCATAGTAATCCTGAGGAGGAAGAATGGCTGACAGCTGAGCGAATGGCAGTAAGCCTCTTAAATCTTTTATTGTGAATAAGACACTGCTT
Recommended Reference Assay​
TERT (DCR0000186), AP3B1 (DCR0000238), RPP30 (DCR0000181), AGO1 (DCR0000536)
Recommended Restriction Enzyme​
CviQI, HaeIII, EcoRI, XbaI
Wet-lab validated​
dPCR wet-lab validated
Hydrolysis Probe​
FAM, ATTO 550, Cy5
Primer Purification​
Desalted
Probe Purification​
HPLC
Reaction size​
300rxns, 500rxns and 1000rxns depending on selected dye

Resources

Application Notes (1)
Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.
Quick-Start Protocols (1)
Brochures & Guides (1)
For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System
Safety Data Sheets (1)
Certificates of Analysis (1)
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