dPCR Copy Number Variation (CNV) Probe Assays

シングルプレックスまたはマルチプレックスdPCRによるコピー数多型とコピー数異常の遺伝子座特異的解析用

200以上のウェットラボで検証済みのアッセイとインシリコアッセイ設計のためのカスタムツール
色素の選択により、1回の反応あたり最大5つのターゲットのマルチプレックスが可能
対象遺伝子、リファレンス、セントロメアリファレンスアッセイが利用可能
すぐに使用できるアッセイは、FFPE、細胞株、gDNAなどのさまざまなサンプルで検証されています
QIAcuityでのシンプルで高速のdPCRワークフロー
QIAcuity Software Suiteを使用した簡便なコピー数データ分析

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GeneGlobe ID: DCG0000196 | Cat. No.: 250210 | dPCR CNV Probe Assays

dPCR CNV Probe Gene of Interest for PRKN

Gene symbol: PRKN
Ensembl Gene ID: ENSG00000185345
dPCR wet-lab validated

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CAT NO./ID 250101

QIAcuity Probe PCR Kit (1 ml)

4x QIAcuity プローブマスターミックス(1mL、1本)、滅菌水（1.9 ml、2本）

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CAT NO./ID 250102

QIAcuity Probe PCR Kit (5 ml)

4x QIAcuity プローブマスターミックス(1mL、5本)、滅菌水（1.9 ml、8本）

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CAT NO./ID 250103

QIAcuity Probe PCR Kit (25 ml)

4x QIAcuity プローブマスターミックス(5mL、5本)、滅菌水（20 ml、4本）

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CAT NO./ID 250111

QIAcuity EG PCR Kit (1 ml)

3x QIAcuity EvaGreen マスターミックス(1mL、1本)、滅菌水（1.9 ml、2本）

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CAT NO./ID 69506

DNeasy Blood & Tissue Kit (250)

250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)

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CAT NO./ID 74106

RNeasy Mini Kit (250)

250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers.

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Resources

Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.

For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System

dPCR Copy Number Variation (CNV) Probe Assays

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dPCR CNV Probe Gene of Interest for PRKN

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Resources