Assay Name
dPCR Mutation Assay EGFR 12382 Human
GeneGlobe Cat No (Assay ID)
DMH0000304
Species
Human (Homo sapiens)
Gene Symbol
EGFR
Gene aliases
ERBB1;ERRP;ERBB
Ensembl Gene ID
ENSG00000146648
Entrez Gene ID
1956
Genomic Mutation ID (COSV by COSMIC)
COSV51765099
Legacy Mutation ID (COSM by COSMIC)
COSM12382
Amino Acid Change
p.L747_A750delinsP
Nucleotide Change
c.2239_2248delinsC
Wildtype Allele
ATTAAGAGAAG
Mutant Allele
AC
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
TTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGG
Amplicon length
142
Recommended Restriction Enzyme
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC