dPCR Mutation Assay ABL1 12576 Human

Product Specification

AA Mutation: p.Y272H
Nucleotide Mutation: c.814T>C
dPCR wet-lab validated

Assay Name

dPCR Mutation Assay ABL1 12576 Human

GeneGlobe Cat No (Assay ID)

DMH0000571

Species

Human (Homo sapiens)

Reaction size

200rxns and 1000rxns

Gene Symbol

ABL1

Gene aliases

JTK7;c-ABL;p150;ABL

Ensembl Gene ID

ENSG00000097007

Entrez Gene ID

Genomic Mutation ID (COSV by COSMIC)

COSV59325175

Legacy Mutation ID (COSM by COSMIC)

COSM12576

Amino Acid Change

p.Y272H

Nucleotide Change

c.814T>C

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

TGGAACGCACGGACATCACCATGAAGCACAAGCTGGGCGGGGGCCAGTACGGGGAGGTGTACGAGGGCGTGTGGAAGAAATACAGCCTGACGGTGGCCGT

Amplicon length

Recommended Restriction Enzyme

EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX or ATTO550/ROX

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

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dPCR Mutation Assay ABL1 12576 Human

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Product Specification

Resources

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