dPCR LNA Mutation Assays

For detecting specific DNA sequence mutations related to cancer and oncogenesis

Duplex assay design detects mutated and wild-type sequences
Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
LNA-enhanced primers and probes increase assay specificity and sensitivity
Wet-lab tested dPCR assays available for more than 200 targets
Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
Intended for use with QIAcuity MasterMix

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Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.