Custom digital PCR assays for high-precision analyses
Create and order with our online dPCR design tools
DPCR CUSTOM ASSAY DESIGNERS
Create high-specificity custom digital PCR assays
Our online design tools let you easily create and order tailor-made dPCR primer and probe sets for detecting targets where no predesigned products are available. These custom dPCR assays are designed for use with QIAcuity PCR kits compatible with hydrolysis probes and the QIAcuity digital PCR instrument. Our dPCR custom design tools currently support the following applications, delivering results with exceptional accuracy, precision and reproducibility.
- Copy Number Variation (CNV) Analysis: Custom dPCR CNV Probe Assays empower you to confidently detect and quantify subtle changes in gene copy numbers, enabling breakthroughs in areas such as cancer research, genetic disorders and developmental biology. These assays provide clear, easy-to-interpret results, allowing you to focus on what matters most - advancing your research.
- Microbial Detection: Custom dPCR Microbial Assays enable precise quantification of bacteria, fungi or viruses, making them ideal for applications in pathogen detection, environment monitoring and microbiome research. With these assays, you can achieve reliable, reproducible data to drive your studies forward with confidence.
Products | Custom dPCR CNV Probe Assays | Custom dPCR Microbial Assays |
|---|---|---|
| Supported Species | Human, mouse, rat | Bacteris 16S, fungi ITS, viruses |
| Detection Chemistry | Hydrolysis probe | Hydrolysis probe |
| Fluorophores | Cy5, FAM, HEX, ROX, ATTO550 | Cy5, FAM, HEX, ROX, TAMRA |
| Format | Tube containing pre-mixed and lyophilized primers and probe | Tube containing pre-mixed and lyophilized primers and probe |
| Pack Size | 75 or 180 x 40 μl reactions (QIAcuity Nanoplate 26k) or 250 or 600 x 12 μl reactions (QIAcuity Nanoplate 8.5k) | 150 or 400 x 40 μl reactions (QIAcuity Nanoplate 26k) or 500 or 1333 x 12 μl reactions (QIAcuity Nanoplate 8.5k) |
| Design Tool Input | Sequences, gene symbols, genomic coordinates | Organism name, species, taxonomy ID |
| Reference Database | Human: GRCh38, Mouse: GRCm39, Rat: mRatBN7.2 | NCBI |
| LNA-Enhanced | No | LNA-enhancement for complex designs |
| Multiplexing | Yes, multiple fluorophores available; no integrated multiplex check | Yes, multiple fluorophores available; no integrated multiplex check |
| Positive Sequence | Sequence information on amplicon region provided allowing the order of a synthetic positive control | Sequence information on amplicon region provided allowing the order of a synthetic positive control |
| Other Features | Option to integrate reference assays into the design process | Option to design against customer-defined anchor sequence |
| Data Analysis | QIAcuity Software Suite | QIAcuity Software Suite |
Stay tuned – more dPCR custom assay design tools coming soon!
Prepare to elevate your digital PCR capabilities to new heights with our soon-to-launch custom assay design tools.