Assay name
HS_CFTR_2474708 QuantiNova LNA PCR Assay
Wet-lab validated
qPCR wet-lab validated
Assay specification
Best coverage assay for CFTR
Best transcript assay for ENST00000649406
Assay targets 5 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Best transcript assay for ENST00000649406
Assay targets 5 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Gene symbol
CFTR
Gene aliases
MRP7;ABC35;TNR-CFTR;dJ760C5.1;CFTR/MRP;CF;ABCC7
Species
Homo sapiens
Main target
ENST00000649406
Exons numbers
4-5
Ensembl exons ids
ENSE00000718611;ENSE00000718620
Amplicon start
522
Amplicon length
77
Assay targets
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENST00000003084
Protein coding
CCDS5773
NM_000492
Chr.7: 117479963 - 117668665
ENST00000647978
Nonsense mediated decay
Chr.7: 117480011 - 117594988
ENST00000648260
Protein coding
Chr.7: 117480011 - 117627910
ENST00000649406
Protein coding
Chr.7: 117480011 - 117627910
ENST00000649781
Protein coding
Chr.7: 117480095 - 117667108