GeneGlobe ID: DMH0000342 | Cat. No.: Varies | dPCR LNA Mutation Assays

dPCR Mutation Assay NRAS 570 Human

Product Specification

AA Mutation: p.G13C
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated
Assay Name​
dPCR Mutation Assay NRAS 570 Human
GeneGlobe Cat No (Assay ID)​
DMH0000342
Species​
Human (Homo sapiens)
Gene Symbol​
NRAS
Gene aliases​
N-ras
Ensembl Gene ID​
ENSG00000213281
Entrez Gene ID​
4893
Genomic Mutation ID (COSV by COSMIC)​
COSV54736386
Legacy Mutation ID (COSM by COSMIC)​
COSM570
Amino Acid Change​
p.G13C
Nucleotide Change​
c.37G>T
Wildtype Allele​
C
Mutant Allele​
A
Mutation Strand​
-
Mutant description​
Substitution - Missense
Control Sequence (wt)​
GGGATCATATTCATCTACAAAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACCTGCTCCAACCACCACCAGTTTGTACTCAGTCATTTCACAC
Amplicon length​
89
Recommended Restriction Enzyme​
HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX, ATTO550/ROX or Cy5/ATTO700
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC
Reaction size​
200rxns and 1000rxns

Product Resources

icon_0245_cc_gen_pdf-s Validation Data
File Size: 100.32 KBLanguage: English

Resources

Available Product Catalog (2)
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Download Safety Data Sheets for QIAGEN product components.
アプリケーションノート (2)
Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Safety Data Sheets (1)
Certificates of Analysis (1)
Application Notes (2)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis
Quick-Start Protocols (1)
Instrument User Manuals (1)
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