dPCR LNA Mutation Assays

用于检测与癌和肿瘤发生有关的特定 DNA 序列突变

双重检测设计，可检测突变型及野生型序列
可选 FAM + HEX 或 Atto 550 + ROX 荧光染料组合
LNA 增强的引物和探针，可提高检测的特异性和灵敏度
经过湿性实验室测试的 dPCR 检测，有 200 多个靶标供选
适合与循环肿瘤 DNA、液体活检、FFPE 和其他组织样本一起使用
旨在与 QIAcuity Probe PCR Kit 一起使用

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Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.