dPCR Mutation Assay PIK3CA 754 Human

AA Mutation: p.N345K
Nucleotide Mutation: c.1035T>A
dPCR wet-lab validated

Assay Name

GeneGlobe Cat No (Assay ID)

DMH0000031

Species

Human (Homo sapiens)

Gene Symbol

PIK3CA

Gene aliases

PI3K

Ensembl Gene ID

ENSG00000121879

Entrez Gene ID

5290

Genomic Mutation ID (COSV by COSMIC)

COSV55873276

Legacy Mutation ID (COSM by COSMIC)

COSM754

Amino Acid Change

p.N345K

Nucleotide Change

c.1035T>A

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

TGAATGGAGAAACATCTACAAAATCCCTTTGGGTTATAAATAGTGCACTCAGAATAAAAATTCTTTGTGCAACCTACGTGAATGTAAATATTCGAGACATTGATAAGGTAAAGTCAAATGCTGA

Amplicon length

104

Recommended Restriction Enzyme

CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

dPCR Mutation Assay PIK3CA 754 Human

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dPCR Mutation Assay PIK3CA 754 Human

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Product Specification

Product Resources

Resources

Select a variant to see the price