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dPCR LNA Mutation Assays

がんやがんの発生に関連した特異的なDNA配列の変異検出のために

  • デュプレックスアッセイ設計により、変異型と野生型の配列を検出
  • FAMとHEXまたはAtto 550とROXの蛍光色素の組み合わせを選択可能
  • LNAを組み込んだプライマーとプローブがアッセイの特異性と感度を向上
  • 200以上のターゲットのdPCRアッセイをウェットラボで検証済み
  • 循環腫瘍DNA、リキッドバイオプシー、FFPE、およびその他の組織サンプルでの使用に最適
  • QIAcuity Probe PCR Kitとの併用を想定

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Product

Product List20 products

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GeneGlobe ID: DMH0000285 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 520 Human
AA Mutation: p.G12V
Nucleotide Mutation: c.35G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000024 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 1135364 Human
AA Mutation: p.Q61H
Nucleotide Mutation: c.183A>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000331 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 528 Human
AA Mutation: p.G13S
Nucleotide Mutation: c.37G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000195 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 527 Human
AA Mutation: p.G13C
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000286 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 521 Human
AA Mutation: p.G12D
Nucleotide Mutation: c.35G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000309 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 516 Human
AA Mutation: p.G12C
Nucleotide Mutation: c.34G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000519 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 517 Human
AA Mutation: p.G12S
Nucleotide Mutation: c.34G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000289 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 532 Human
AA Mutation: p.G13D
Nucleotide Mutation: c.38G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000290 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 1159597 Human
AA Mutation: p.Q61K
Nucleotide Mutation: c.181C>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000334 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 533 Human
AA Mutation: p.G13A
Nucleotide Mutation: c.38G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000525 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 531 Human
AA Mutation: p.G13D
Nucleotide Mutation: c.38_39delinsAT
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000022 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 551 Human
AA Mutation: p.Q61P
Nucleotide Mutation: c.182A>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000025 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 1146992 Human
AA Mutation: p.Q61H
Nucleotide Mutation: c.183A>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001055 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 522 Human
AA Mutation: p.G12A
Nucleotide Mutation: c.35G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000198 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 553 Human
AA Mutation: p.Q61L
Nucleotide Mutation: c.182A>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000332 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 529 Human
AA Mutation: p.G13R
Nucleotide Mutation: c.37G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000044 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 550 Human
AA Mutation: p.Q61E
Nucleotide Mutation: c.181C>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000527 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 534 Human
AA Mutation: p.G13V
Nucleotide Mutation: c.38G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000284 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 518 Human
AA Mutation: p.G12R
Nucleotide Mutation: c.34G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000023 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay KRAS 552 Human
AA Mutation: p.Q61R
Nucleotide Mutation: c.182A>G
dPCR wet-lab validated
Product Specification
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Resources

サイエンティフィック・ポスター (2)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
機器ユーザーマニュアル (1)
QIAcuity Application Guide
PDF (4MB)
パンフレット (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
クイックスタートプロトコール (1)
dPCR LNA Mutation Assays
PDF (175KB)
MSDS (1)
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
Application Notes (1)
Liquid biopsy-based detection of PIK3CA mutations from cfDNA using an end-to-end digital PCR workflow
PDF (2MB)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Safety Data Sheets (1)
Safety Data Sheets
Certificates of Analysis (1)
Certificates of Analysis
Brochures & Guides (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
Scientific Posters (2)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Quick-Start Protocols (1)
dPCR LNA Mutation Assays
PDF (175KB)
Instrument User Manuals (1)
QIAcuity Application Guide
PDF (4MB)
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