dPCR Mutation Assay TP53 10714 Human

AA Mutation: p.R158L
Nucleotide Mutation: c.473G>T
dPCR wet-lab validated

Assay Name

GeneGlobe Cat No (Assay ID)

DMH0000362

Species

Human (Homo sapiens)

Gene Symbol

TP53

Gene aliases

p53;LFS1

Ensembl Gene ID

ENSG00000141510

Entrez Gene ID

7157

Genomic Mutation ID (COSV by COSMIC)

COSV52676395

Legacy Mutation ID (COSM by COSMIC)

COSM10714

Amino Acid Change

p.R158L

Nucleotide Change

c.473G>T

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

CACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGCGGGGGTGTGGAATCAACCCACAGCTGCACAGGGCAGGTCT

Amplicon length

Recommended Restriction Enzyme

CviQI,EcoRI,XbaI

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

dPCR Mutation Assay TP53 10714 Human

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dPCR Mutation Assay TP53 10714 Human

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Product Specification

Product Resources

Resources

Select a variant to see the price