dPCR Mutation Assay KRAS 522 Human

AA Mutation: p.G12A
Nucleotide Mutation: c.35G>C
dPCR wet-lab validated

Assay Name

GeneGlobe Cat No (Assay ID)

DMH0001055

Species

Human (Homo sapiens)

Gene Symbol

KRAS

Gene aliases

KRAS1;K-Ras4B;KRAS2

Ensembl Gene ID

ENSG00000133703

Entrez Gene ID

3845

Genomic Mutation ID (COSV by COSMIC)

COSV55497479

Legacy Mutation ID (COSM by COSMIC)

COSM522

Amino Acid Change

p.G12A

Nucleotide Change

c.35G>C

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACCACAAGTTTATATTCAGTCATTTTCAGCAGGCCTTATAATAAAAA

Amplicon length

165

Recommended Restriction Enzyme

CviQI,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

dPCR Mutation Assay KRAS 522 Human

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Product Specification

Resources

dPCR Mutation Assay KRAS 522 Human

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Product Specification

Resources

Select a variant to see the price