AA Mutation: p.N345K
Nucleotide Mutation: c.1035T>A
dPCR wet-lab validated
Nucleotide Mutation: c.1035T>A
dPCR wet-lab validated
Assay Name
dPCR Mutation Assay PIK3CA 754 Human
GeneGlobe Cat No (Assay ID)
DMH0000031
Species
Human (Homo sapiens)
Gene Symbol
PIK3CA
Gene aliases
PI3K
Ensembl Gene ID
ENSG00000121879
Entrez Gene ID
5290
Genomic Mutation ID (COSV by COSMIC)
COSV55873276
Legacy Mutation ID (COSM by COSMIC)
COSM754
Amino Acid Change
p.N345K
Nucleotide Change
c.1035T>A
Wildtype Allele
T
Mutant Allele
A
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
TGAATGGAGAAACATCTACAAAATCCCTTTGGGTTATAAATAGTGCACTCAGAATAAAAATTCTTTGTGCAACCTACGTGAATGTAAATATTCGAGACATTGATAAGGTAAAGTCAAATGCTGA
Amplicon length
104
Recommended Restriction Enzyme
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX, ATTO550/ROX or Cy5/ATTO700
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC
Reaction size
200rxns and 1000rxns