dPCR CNV Probe Gene of Interest for IL17RA

Product Specification

Gene symbol: IL17RA
Ensembl Gene ID: ENSG00000177663
dPCR wet-lab validated

Product Name

dPCR CNV Probe Gene of Interest for IL17RA

GeneGlobe Cat.No. (Assay ID)

DCG0000194

Assay type

Gene of Interest

Species

Human (Homo sapiens)

Gene symbol

IL17RA

Ensembl Gene ID

ENSG00000177663

NCBI Gene Id

23765

Strand

Binds to forward (5’ to 3’) top genome strand

Amplicon length

Amplicon region

GTGCACCTGCGCTTCCTGTCGAGAACATTCATTATGCAAAAGTCAGGAAAGGAGAAACAGAACTGTCATTTGGATTGTGAAAGTATTCTCTGGGGAGCTGCCCTAC

Recommended Reference Assay

TERT (DCR0000186), AP3B1 (DCR0000238), RPP30 (DCR0000181), AGO1 (DCR0000536)

Recommended Restriction Enzyme

CviQI, AluI, HaeIII, EcoRI, XbaI, PvuII

Wet-lab validated

dPCR wet-lab validated

Hydrolysis Probe

FAM, ATTO 550, Cy5

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

300rxns, 500rxns and 1000rxns depending on selected dye

Resources

Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.

For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System

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dPCR CNV Probe Gene of Interest for IL17RA

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Product Specification

Resources

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