Assay Nameβ
dPCR Mutation Assay EGFR 12419 Human
GeneGlobe Cat No (Assay ID)β
DMH0000075
Speciesβ
Human (Homo sapiens)
Gene Symbolβ
EGFR
Gene aliasesβ
ERBB1;ERRP;ERBB
Ensembl Gene IDβ
ENSG00000146648
Entrez Gene IDβ
1956
Genomic Mutation ID (COSV by COSMIC)β
COSV51863059
Legacy Mutation ID (COSM by COSMIC)β
COSM12419
Amino Acid Changeβ
p.L747_T751delinsQ
Nucleotide Changeβ
c.2238_2252delinsGCA
Wildtype Alleleβ
AATTAAGAGAAGCAAC
Mutant Alleleβ
AGCA
Mutation Strandβ
+
Mutant descriptionβ
Substitution - Missense
Positive Control Sequenceβ
TAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGG
Amplicon lengthβ
109
Recommended Restriction Enzymeβ
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validatedβ
dPCR wet-lab validated
Probe Fluorophoreβ
FAM/HEX or ATTO550/ROX
Quencherβ
Iowa Black
Primer Purificationβ
Desalted
Probe Purificationβ
HPLC