dPCR LNA Mutation Assays

For detecting specific DNA sequence mutations related to cancer and oncogenesis

  • Duplex assay design detects mutated and wild-type sequences
  • Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
  • LNA-enhanced primers and probes increase assay specificity and sensitivity
  • Wet-lab tested dPCR assays available for more than 200 targets
  • Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
  • Intended for use with QIAcuity Probe PCR Kits

Product

Product List17 products

GeneGlobe ID: DMH0000510 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 571 Human
AA Mutation: p.G13S
Nucleotide Mutation: c.37G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000188 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 563 Human
AA Mutation: p.G12S
Nucleotide Mutation: c.34G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000336 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 561 Human
AA Mutation: p.G12R
Nucleotide Mutation: c.34G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000340 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 566 Human
AA Mutation: p.G12V
Nucleotide Mutation: c.35G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000183 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 584 Human
AA Mutation: p.Q61R
Nucleotide Mutation: c.182A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000343 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 573 Human
AA Mutation: p.G13D
Nucleotide Mutation: c.38G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000339 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 565 Human
AA Mutation: p.G12A
Nucleotide Mutation: c.35G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000341 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 569 Human
AA Mutation: p.G13R
Nucleotide Mutation: c.37G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000344 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 574 Human
AA Mutation: p.G13V
Nucleotide Mutation: c.38G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000345 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 575 Human
AA Mutation: p.G13A
Nucleotide Mutation: c.38G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000349 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 585 Human
AA Mutation: p.Q61H
Nucleotide Mutation: c.183A>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000342 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 570 Human
AA Mutation: p.G13C
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000186 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 562 Human
AA Mutation: p.G12C
Nucleotide Mutation: c.34G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000347 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 581 Human
AA Mutation: p.Q61E
Nucleotide Mutation: c.181C>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000505 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 580 Human
AA Mutation: p.Q61K
Nucleotide Mutation: c.181C>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000180 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 586 Human
AA Mutation: p.Q61H
Nucleotide Mutation: c.183A>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000190 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 583 Human
AA Mutation: p.Q61L
Nucleotide Mutation: c.182A>T
dPCR wet-lab validated
Product Specification
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Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)