dPCR Copy Number Variation (CNV) Probe Assays

For locus-specific analysis of copy number variations and alterations by singleplex or multiplex dPCR

More than 200 wet-lab-validated assays and a custom tool for in-silico assay design
Dye selection enables multiplexing of up to 5 targets per reaction
Gene of interest, reference and centromeric reference assays available
Ready-to-use assays have been validated for a range of samples, such as FFPE, cell lines and gDNA
Simple and fast dPCR workflow on the QIAcuity
Convenient copy number data analysis using the QIAcuity Software Suite

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Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.

For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System