Assay name​
HS_DHFR_1751249 QuantiNova LNA PCR Assay
Assay specification​
Best coverage assay for DHFR
Assay targets 7 transcripts
Assay spans exon
IMPORTANT: May detect gDNA
Pre-designed assay for dPCR and qPCR.
Assay targets 7 transcripts
Assay spans exon
IMPORTANT: May detect gDNA
Pre-designed assay for dPCR and qPCR.
Gene symbol​
DHFR
Species​
Homo sapiens
Main target​
ENST00000513314
Exons numbers​
1-2
Ensembl exons ids​
ENSE00002065031;ENSE00002054951
Amplicon start​
27
Amplicon length​
64
Assay targets​
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENST00000439211
Protein coding
CCDS47240
NM_000791
Chr.5: 80626226 - 80654983
ENST00000504396
Protein coding
CCDS78028
NM_001290354
Chr.5: 80628223 - 80654558
ENST00000505337
Protein coding
CCDS47240
Chr.5: 80628017 - 80654674
ENST00000508282
Retained intron
Chr.5: 80629064 - 80654101
ENST00000511032
Protein coding
CCDS78029
NM_001290357
Chr.5: 80628124 - 80654552
ENST00000513048
Processed transcript
Chr.5: 80628538 - 80654547
ENST00000513314
Retained intron
Chr.5: 80648941 - 80654035