Assay name​
MM_SPARC_2011627 QuantiNova LNA PCR Assay
Wet-lab validated​
qPCR wet-lab validated
Assay specification​
Best coverage assay for Sparc
Assay targets 7 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Assay targets 7 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Gene symbol​
Sparc
Species​
Mus musculus
Main target​
ENSMUST00000108858
Exons numbers​
6-7
Ensembl exons ids​
ENSMUSE00000448017;ENSMUSE00000448024
Amplicon start​
542
Amplicon length​
63
Assay targets​
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENSMUST00000018737
Protein coding
CCDS24712
NM_009242
Chr.11: 55394500 - 55420080
ENSMUST00000108858
Protein coding
CCDS70189
NM_001290817
Chr.11: 55394500 - 55419898
ENSMUST00000130642
Retained intron
Chr.11: 55395520 - 55402165
ENSMUST00000141530
Protein coding
Chr.11: 55399252 - 55419898
ENSMUST00000213866
Protein coding
Chr.11: 55395421 - 55420290
ENSMUST00000214685
Protein coding
CCDS24712
Chr.11: 55395421 - 55423183
ENSMUST00000216313
Protein coding
Chr.11: 55395421 - 55420325