Assay name​
MM_HIF1A_2247755 QuantiNova LNA PCR Assay
Wet-lab validated​
qPCR wet-lab validated
Assay specification​
Best coverage assay for Hif1a
Assay targets 4 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Assay targets 4 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Gene symbol​
Hif1a
Species​
Mus musculus
Main target​
ENSMUST00000110464
Exons numbers​
2-3
Ensembl exons ids​
ENSMUSE00001223008;ENSMUSE00000114679
Amplicon start​
575
Amplicon length​
90
Assay targets​
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENSMUST00000021530
Protein coding
CCDS25977
NM_001313919;NM_010431
Chr.12: 73907904 - 73947530
ENSMUST00000110461
Protein coding
CCDS83968
NM_001313920
Chr.12: 73901375 - 73947530
ENSMUST00000110464
Protein coding
Chr.12: 73907904 - 73933076
ENSMUST00000149740
Retained intron
Chr.12: 73926602 - 73928532