Assay Name
dPCR Mutation Assay JAK2 12600 Human
GeneGlobe Cat No (Assay ID)
DMH0000021
Species
Human (Homo sapiens)
Gene Symbol
JAK2
Gene aliases
JTK10
Ensembl Gene ID
ENSG00000096968
Entrez Gene ID
3717
Genomic Mutation ID (COSV by COSMIC)
COSV67569051
Legacy Mutation ID (COSM by COSMIC)
COSM12600
Amino Acid Change
p.V617F
Nucleotide Change
c.1849G>T
Wildtype Allele
G
Mutant Allele
T
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
AGTCTTTCTTTGAAGCAGCAAGTATGATGAGCAAGCTTTCTCACAAGCATTTGGTTTTAAATTATGGAGTATGTGTCTGTGGAGACGAGAGTAAGTAAAACTACAGGCTTTCTAATGCCTT
Amplicon length
101
Recommended Restriction Enzyme
CviQI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC