dPCR Mutation Assay PIK3CA 764 Human

Product Specification

AA Mutation: p.E545G
Nucleotide Mutation: c.1634A>G
dPCR wet-lab validated

Assay Name

dPCR Mutation Assay PIK3CA 764 Human

GeneGlobe Cat No (Assay ID)

DMH0000033

Species

Human (Homo sapiens)

Gene Symbol

PIK3CA

Gene aliases

PI3K

Ensembl Gene ID

ENSG00000121879

Entrez Gene ID

5290

Genomic Mutation ID (COSV by COSMIC)

COSV55873220

Legacy Mutation ID (COSM by COSMIC)

COSM764

Amino Acid Change

p.E545G

Nucleotide Change

c.1634A>G

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

AGCTCAAAGCAATTTCTACACGAGATCCTCTCTCTGAAATCACTGAGCAGGAGAAAGATTTTCTATGGAGTCACAGGTAAGTGCTAAAATG

Amplicon length

Recommended Restriction Enzyme

CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis

Introducing the dPCR Mutation Assay PIK3CA 764 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Mutation/Variant Detection applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay PIK3CA 764 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Mutation/Variant Detection, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay PIK3CA 764 Human and elevate your work to new heights.

Country and Language

dPCR Mutation Assay PIK3CA 764 Human

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Product Specification

Resources

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