Assay Name
dPCR Mutation Assay TP53 10808 Human
GeneGlobe Cat No (Assay ID)
DMH0000112
Species
Human (Homo sapiens)
Gene Symbol
TP53
Gene aliases
p53;LFS1
Ensembl Gene ID
ENSG00000141510
Entrez Gene ID
7157
Genomic Mutation ID (COSV by COSMIC)
COSV52663142
Legacy Mutation ID (COSM by COSMIC)
COSM10808
Amino Acid Change
p.Y163C
Nucleotide Change
c.488A>G
Wildtype Allele
T
Mutant Allele
C
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
AGCGCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGCGGGGGTGTGGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACATCTTGTT
Amplicon length
116
Recommended Restriction Enzyme
CviQI,EcoRI,XbaI
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC