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GeneGlobe ID: PAHS-030Z | Cat. No.: 330231 | RT2 Profiler PCR Arrays

RT² Profiler™ PCR Array Human Insulin Signaling Pathway

RT2 Profiler PCR Array

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Product Specification

Target List

Insulin Receptor-Associated Proteins
Insulin & Receptors​
INSINSL3IRS1IRS2IRS4SORBS1
Insulin-Like Growth Factors & Receptors​
IGF1RIGF2IGFBP1
SH3 / SH2 Adaptor Proteins​
CBLGAB1GRB2IRS4NCK1
Other Insulin Receptor-Associated Proteins​
CAP1DOK1DOK2DOK3FRS2FRS3INSRPDPK1PPP1CAPTPN1 (PTP1B)PTPRFSHC1
PI3 Kinase Signaling
PI3 Kinase Canoncial Pathway Genes​
AKT1AKT2AKT3EIF2B1EIF4EBP1 (4E-BP1)GSK3AGSK3BMTORPDPK1PIK3CA (p110-alpha)PIK3R1 (p85-ALPHA)PIK3R2 (p85-BETA)PRKCGPRKCIPRKCZ
PI3 Kinase Signaling Target Genes​
BCL2L1 (BCLXL)BCL2L1 (BCLXL)BCL2L1 (BCLXL)DUSP14G6PCHK2IGFBP1PCK2SERPINE1 (PAI-1)SLC2A4 (GLUT4)SREBF1UCP1VEGFA
MAP Kinase Signaling
MAP Kinase Canoncial Pathway Genes​
ARAFBRAFEIF2B1GRB2HRASMAP2K1MAPK1 (ERK2)RAF1RPS6KA1RRASRRAS2SHC1SOS1
MAP Kinase Signaling Target Genes​
ANGBCL2L1 (BCLXL)BCL2L1 (BCLXL)BCL2L1 (BCLXL)ERCC1FOSLDLRNOS2 (iNOS)UCP1
Primary Insulin Signaling Target Genes
CEBPACEBPBFOSJUNLEP (Leptin)PRL
Secondary Insulin Signaling Effector Target Genes
NPYPCK2SLC2A1SLC2A4 (GLUT4)TG
PPARγ Targets
ACOX1ADRB3AEBP1CAP1CEBPACEBPBPCK2PPARGRETN
SREBP1 Targets
ACACAFBP1G6PCGCKPCK2PKM
Carbohydrate Metabolism
Glucose Metabolism​
FBP1G6PCGCKHK2INSIRS2PCK2PKMSLC2A1SLC2A4 (GLUT4)SORBS1
Glycogen Metabolism​
G6PCGSK3BPPP1CA
Other Carbohydrate Metabolism Genes​
ADRB3GPD1INSR
Lipid Metabolism
Cholesterol Metabolism​
LDLRSREBF1
Fatty Acid Metabolism​
ACACAACOX1FASN
Lipid Transport​
LDLRSORBS1
Other Lipid Metabolism Genes​
ARAFBRAFINSLEP (Leptin)PPARGPRKCGPRKCIPRKCZRAF1SHC1
Protein Metabolism
Protein Phosphatases​
DUSP14PPP1CAPTPN1 (PTP1B)PTPRF
Protein Kinases​
AKT1AKT2AKT3ARAFBRAFGSK3AGSK3BIGF1RINSL3INSRMAP2K1MAPK1 (ERK2)PDPK1PRKCGPRKCIPRKCZRAF1RPS6KA1
Protein Biosynthesis​
ANGEIF2B1EIF4EBP1 (4E-BP1)LDLRPPP1CA
Protein Transport​
PRKCIRRAS2
Other Protein Metabolism Genes​
AEBP1CBLDOK1DOK2FRS2GAB1IRS1NOS2 (iNOS)SORBS1VEGFA
Transcription Factors & Regulators
Transcriptional Regulation​
CEBPACEBPBFOSJUNPPARGSREBF1
Transcription Factors​
AEBP1CBLCEBPACEBPBFOSJUNPPARGSREBF1
Cell Growth & Differentiation
Cell Cycle​
HK2HRASIGF1RIGF2KRASMAPK1 (ERK2)MTORVEGFA
Cell Proliferation​
GAB1IGF1RIGF2IRS2NCK1NPYPRLRAF1SHC1VEGFA
Growth Factors & Receptors​
GAB1GRB2IGF1RIGF2IGFBP1INSRSHC1VEGFA
Cell Differentiation​
NOS2 (iNOS)PPARG

Product Resources

icon_0041_download-s Download Target List
icon_0245_cc_gen_pdf-s Array Layout for PCR Plates
File Size: 138.31 KBLanguage: English
icon_0245_cc_gen_pdf-s Array Layout for Rotor-Gene Q
File Size: 132 KBLanguage: English

Resources

Supplementary Protocols (10)
Bio-Rad CFX96 and CFX384 instrument setup instructions for RT2 Profiler PCR Arrays
PDF (298KB)
Roche LightCycler 480 real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (1MB)
Eppendorf Mastercycler ep realplex instrument setup instructions for RT2 Profiler PCR Arrays
PDF (554KB)
ABI 7900HT (for SDS Software 2.1, 2.3 and 2.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (320KB)
Stratagene Mx3000P qPCR System real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (259KB)
Rotor-Gene Q real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (175KB)
ABI 7500 & ABI 7500 FAST (Software Version 2.0.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (388KB)
Bio-Rad iCycler & iQ Real-Time PCR Systems (for Software Version 3.1) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (249KB)
ABI StepOnePlus (for Software Version 2.0) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (77KB)
Life Technologies ViiA7 (ViiA 7 Software v1.2) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (511KB)
Brochures & Guides (3)
Technical Guide to QIAGEN PCR Arrays
PDF (2MB)
RT2 Profiler PCR Arrays: Pathway Analysis - (EN)
PDF (1MB)
Total RNA Discovery - (EN)
PDF (255KB)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
Safety Data Sheets (1)
Safety Data Sheets
Articles (17)
(EN)Managing mortality: how cells regulate telomerase activity
(EN) The role of inflammation in cancer
(EN)A good transcription factor gone rogue: the two faces of NRF2 in oxidative stress
(EN)Metabolic homeostasis: regulation of FOXO by class IIa HDACs
(EN) Chemokines: manipulators of metastasis
(EN) Cellular stress, TGF-β, and EMT: what are the true origins of fibrosis?
(EN) Regulation of BAX mediated apoptosis by BCL2 family members
(EN)Inflammation and neurodegenerative disease: a role for the estrogen receptor in suppression of inflammation in the brain
(EN) Control of PPARγ and mTOR in insulin resistance
(EN) What enhances the inflammatory response: new players and unexpected roles
(EN) DNA damage repair — regulation through autophagy
(EN) Lipid autophagy in obesity and atherosclerosis: chewing the fat
(EN) Induced pluripotent stem cells: real stem cells?
(EN) Silencing cell-cell communication to combat drug toxicity
(EN) miRNA regulation of angiogenesis: new roles for IGF-1R signaling and heparin
(EN) Master Regulators Direct TGF-beta and Smad Signaling in Different Cell Types
(EN)Regulation of the extracellular matrix by miRNA: impact on cancer metastases
Download Files (3)
RT2 Profiler PCR Array Data Analysis v3.5 Handbook
PDF (770KB)
For analyzing gene expression data from RT2 Profiler PCR Arrays 
Housekeeping Gene Data Analysis
XLSX (60KB)
Data analysis file for RT² Profiler PCR Array Housekeeping Genes
Catalog number- 330231
Pathway number- PAXX-000
RNA QC Data Analysis
XLS (484KB)

Data analysis file for RT² ProfilerRT² Profiler™ PCR Array RT2 RNA QC
Catalog number- 330231
Pathway number- PAXX-999

Scientific Posters (1)
Explore the RNA Universe!
PDF (1MB)
Poster for download
Kit Handbooks (1)
RT2 Profiler PCR Array Handbook
PDF (702KB)
For pathway-focused gene expression profiling using real-time RT-PCR
Performance Data (7)
RT2 Profiler Housekeeping Genes PCR Array Data Analysis 1808
XLSX (65KB)
PCR_Array_4x96_384-Well_Conversion Spreadsheet
XLSX (38KB)
RT2 Profiler PCR Array Data Analysis Spreadsheet 1904
XLSX (3MB)
RT2 Profiler RNA QC PCR Array Data Analysis Spreadsheet 1808
XLSX (60KB)
Universal Custom PCR Array Conversion
XLSX (291KB)
RT2 qPCR Assay Data Analysis 1808 Spreadsheet
XLSX (104KB)
RT2 Profiler PCR Array 384HT Data Analysis Spreadsheet 1808
XLSX (1MB)
Instrument Technical Documents (2)
QIAGEN Service Core - (EN)
PDF (333KB)
For gene expression and genomic analysis
(EN) - RT2 Profiler PCR Arrays
PDF (431KB)
For pathway-focused gene expression analysis
White Papers (2)
RT2 Profiler PCR Arrays: Pathway-focused gene expression profiling with qRT-PCR
PDF (1MB)
RT2 Profiler PCR Array application examples
PDF (563KB)
Gene Expression Analysis (1)
(EN) Wnt Signaling: GSK3 Inhibition Through Sequestration in Multivesicular Bodies
Certificates of Analysis (1)
Certificates of Analysis
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