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dPCR LNA Mutation Assays

For detecting specific DNA sequence mutations related to cancer and oncogenesis

  • Duplex assay design detects mutated and wild-type sequences
  • Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
  • LNA-enhanced primers and probes increase assay specificity and sensitivity
  • Wet-lab tested dPCR assays available for more than 200 targets
  • Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
  • Intended for use with QIAcuity Probe PCR Kits

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Product List31 products

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GeneGlobe ID: DMH0000371 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10817 Human
AA Mutation: p.R249S
Nucleotide Mutation: c.747G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000373 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 11063 Human
AA Mutation: p.M237I
Nucleotide Mutation: c.711G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000108 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10742 Human
AA Mutation: p.H193R
Nucleotide Mutation: c.578A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000131 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 99626 Human
AA Mutation: p.C238F
Nucleotide Mutation: c.713G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000090 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10733 Human
AA Mutation: p.Q192*
Nucleotide Mutation: c.574C>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000440 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10758 Human
AA Mutation: p.Y220C
Nucleotide Mutation: c.659A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000362 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10714 Human
AA Mutation: p.R158L
Nucleotide Mutation: c.473G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000382 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 342245 Human
AA Mutation: p.G154V
Nucleotide Mutation: c.461G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000475 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 220778 Human
AA Mutation: p.R158H
Nucleotide Mutation: c.473G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000119 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 129857 Human
AA Mutation: p.P152L
Nucleotide Mutation: c.455C>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000376 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 11218 Human
AA Mutation: p.T155N
Nucleotide Mutation: c.464C>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000112 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10808 Human
AA Mutation: p.Y163C
Nucleotide Mutation: c.488A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000434 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10739 Human
AA Mutation: p.A161T
Nucleotide Mutation: c.481G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000470 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 43687 Human
AA Mutation: p.H214R
Nucleotide Mutation: c.641A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000375 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 11196 Human
AA Mutation: p.G245V
Nucleotide Mutation: c.734G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000120 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 131481 Human
AA Mutation: p.V157F
Nucleotide Mutation: c.469G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000372 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10863 Human
AA Mutation: p.P278L
Nucleotide Mutation: c.833C>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000380 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 43871 Human
AA Mutation: p.R249M
Nucleotide Mutation: c.746G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000094 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10660 Human
AA Mutation: p.R273H
Nucleotide Mutation: c.818G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000448 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 6932 Human
AA Mutation: p.G245S
Nucleotide Mutation: c.733G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000381 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 6549 Human
AA Mutation: p.R248L
Nucleotide Mutation: c.743G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000364 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 1645467 Human
AA Mutation: p.E286K
Nucleotide Mutation: c.856G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000463 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 43947 Human
AA Mutation: p.Y205C
Nucleotide Mutation: c.614A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000114 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 3675521 Human
AA Mutation: p.R273L
Nucleotide Mutation: c.818G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000361 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10710 Human
AA Mutation: p.E298*
Nucleotide Mutation: c.892G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000377 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 43629 Human
AA Mutation: p.R249W
Nucleotide Mutation: c.745A>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000104 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10891 Human
AA Mutation: p.V272M
Nucleotide Mutation: c.814G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000471 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10810 Human
AA Mutation: p.C242F
Nucleotide Mutation: c.725G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000353 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 10645 Human
AA Mutation: p.C176F
Nucleotide Mutation: c.527G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000374 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 11081 Human
AA Mutation: p.G245C
Nucleotide Mutation: c.733G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000126 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay TP53 43559 Human
AA Mutation: p.V173L
Nucleotide Mutation: c.517G>T
dPCR wet-lab validated
Product Specification
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Resources

Scientific Posters (2)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Instrument User Manuals (1)
QIAcuity Application Guide
PDF (4MB)
Brochures & Guides (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
Quick-Start Protocols (1)
dPCR LNA Mutation Assays
PDF (175KB)
Safety Data Sheets (1)
Safety Data Sheets
Application Notes (1)
Liquid biopsy-based detection of PIK3CA mutations from cfDNA using an end-to-end digital PCR workflow
PDF (2MB)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Certificates of Analysis
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