Accuracy: Innovative UMIs (unique molecular indices) eliminate PCR duplication and amplification bias to deliver the most accurate results (see figure Unbiased and accurate gene quantification).
Specificity: The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees high specificity and accurate results (see figure Proprietary primer design delivers gene-specific amplicons – 97% specificity).
Uniformity: The QIAseq Targeted RNA Panel workflow has been optimized to deliver highly uniform sequencing results, to ensure sequencing capacity is utilized very efficiently. In fact, UMIs (unique molecular indices) entirely remove PCR bias in RNAseq counting (see figure Unmatched uniformity – 97% of assays are within 20% of median molecular tag counts).
Reproducibility: The QIAseq Targeted RNA Panel system demonstrates strong correlations across technical replicates, product lots and instruments with correlation coefficients averaging above >0.99, to ensure reliable detection of differences in expression between biological samples
Sensitivity: Targeted RNA sequencing using UMIs is optimized to deliver highly reliable quantification down to ~100 copies of an RNA target in 25 ng total RNA (see figure Positive results with as little as 0.2 copies of RNA per cell). Low-input protocols allow researchers to start with lower amounts of RNA in smaller volumes.
Flexibility: QIAseq Targeted RNA Panels combine the power of NGS with the accuracy of qPCR to allow multiplexing of several samples per NGS while delivering cost-effective results (see figure Simple procedure).

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QIAseq Targeted RNA Human Immuno-Oncology Panel

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