Nos3 Gene Summary [Mouse]
Enables nitric-oxide synthase activity and scaffold protein binding activity. Involved in several processes, including heart development; negative regulation of muscle hyperplasia; and smooth muscle hyperplasia. Acts upstream of with a positive effect on positive regulation of gene expression. Acts upstream of or within several processes, including lipopolysaccharide-mediated signaling pathway; nitric oxide biosynthetic process; and regulation of metal ion transport. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; cardiovascular system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study aortic valve disease; essential hypertension; persistent fetal circulation syndrome; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); eye disease (multiple); leukemia (multiple); reproductive organ cancer (multiple); and vasculitis (multiple). Orthologous to human NOS3 (nitric oxide synthase 3). [provided by Alliance of Genome Resources, Jul 2025]
Details
| Type | Protein Coding |
| Official Symbol | Nos3 |
| Official Name | nitric oxide synthase 3, endothelial cell [Source:MGI Symbol;Acc:MGI:97362] |
| Ensembl ID | ENSMUSG00000028978 |
| Bio databases IDs | |
| Aliases | nitric oxide synthase 3, endothelial cell |
| Synonyms | 2310065A03Rik, ECNOS, eNOS, nitric oxide synthase 3, nitric oxide synthase 3, endothelial cell |
| Species | Mouse, Mus musculus |
| Orthologies |
Protein Domains
A protein domain is a distinct structural or functional region within a protein that can evolve, function, and exist independently of the rest of the protein chain. These domains in mouse Nos3 often fold into stable, three-dimensional structures and are associated with specific biological functions, such as binding to DNA, other proteins, or small molecules.
- FNR_like
- FAD binding domain
- Nitric oxide synthase, oxygenase domain
- heat shock protein binding
- enzyme
- NADPH-dependent FMN reductase
- NOS_oxygenase
- actin binding
- cadherin binding
- oxygenase domain
- calmodulin binding
- beta-catenin binding
- calmodulin binding domain
- nitric-oxide synthase
- flavodoxin
- enzyme binding
- heme binding domain
- protein binding
- heme binding
- superoxide-generating NADPH oxidase
- phosphorylation site
- amino acid binding
- reductase domain
- binding protein
- actin monomer binding
- hinge domain
- Oxidoreductase NAD-binding domain
Pathways
Biological processes and signaling networks where the Nos3 gene in mouse plays a role, providing insight into its function and relevance in health or disease.
- Adrenomedullin signaling pathway
- AMPK Signaling
- Angiopoietin Signaling
- Apelin Cardiac Fibroblast Signaling Pathway
- Apelin Cardiomyocyte Signaling Pathway
- Apelin Endothelial Signaling Pathway
- Apelin Muscle Signaling Pathway
- Cellular Effects of Sildenafil (Viagra)
- Corticotropin Releasing Hormone Signaling
- Endocannabinoid Cancer Inhibition Pathway
- Endothelin-1 Signaling
- eNOS Signaling
- Erythropoietin Signaling
- Estrogen Receptor Signaling
- Glucocorticoid Receptor Signaling
- Hypoxia Signaling in the Cardiovascular System
- Inhibition of Angiogenesis by TSP1
- Nitric Oxide Signaling in the Cardiovascular System
- Opioid Signaling Pathway
- Relaxin Signaling
- VEGF Family Ligand-Receptor Interactions
Top Findings
The most significant associations for this gene, including commonly observed domains, pathway involvement, and functional highlights based on current data.
disease
- Crohn disease
- atopic dermatitis
- ulcerative colitis
- proteinuria
- stroke
- hypertension
- essential hypertension
- myocardial infarction
- albuminuria
- Alzheimer disease
phenotypes
- abnormal epididymis morphology
- abnormal kidney morphology
- abnormal lymph node morphology
- abnormal spleen morphology
- decreased fasting circulating glucose level
- decreased kidney weight
- enlarged epididymis
- enlarged lymph nodes
- enlarged spleen
- increased heart weight
- increased mean platelet volume
- small kidney
- abnormal adipose tissue amount
- abnormal angiogenesis
- abnormal associative learning
- abnormal blood circulation
- abnormal blood flow velocity
- abnormal blood homeostasis
- abnormal blood vessel physiology
- abnormal blood-brain barrier function
- abnormal bone mineralization
- abnormal cardiac muscle relaxation
- abnormal cardiovascular system morphology
- abnormal cardiovascular system physiology
- abnormal circulating glucose level
- abnormal circulating protein level
- abnormal dentate gyrus morphology
- abnormal dopamine level
- abnormal ejaculation
- abnormal eye physiology
- abnormal female meiosis
- abnormal femur morphology
- abnormal fertility/fecundity
- abnormal glomerular capillary endothelium morphology
- abnormal glucose homeostasis
- abnormal heart left ventricle morphology
- abnormal heart morphology
- abnormal heart right ventricle pressure
- abnormal heart weight
- abnormal hepatocyte morphology
- abnormal induced retinal neovascularization
- abnormal interventricular septum membranous part morphology
- abnormal interventricular septum morphology
- abnormal kidney cortex morphology
- abnormal learning/memory/conditioning
- abnormal leukocyte adhesion
- abnormal limb morphology
- abnormal liver morphology
- abnormal liver physiology
- abnormal long bone epiphyseal plate morphology
- abnormal long term object recognition memory
- abnormal lung morphology
- abnormal lung vasculature morphology
- abnormal myocardial fiber physiology
- abnormal nervous system physiology
- abnormal neuron morphology
- abnormal neuronal precursor proliferation
- abnormal neutrophil physiology
- abnormal oocyte morphology
- abnormal oogenesis
- abnormal osteoblast physiology
- abnormal osteoclast physiology
- abnormal ovary morphology
- abnormal ovary physiology
- abnormal ovulation
- abnormal penile erection
- abnormal pregnancy
- abnormal primary polar body morphology
- abnormal proximal convoluted tubule morphology
- abnormal pulmonary alveolus morphology
- abnormal pulmonary artery morphology
- abnormal pulmonary pressure
- abnormal pulmonary vascular resistance
- abnormal redox activity
- abnormal renal glomerulus morphology
- abnormal renal tubule epithelium morphology
- abnormal response/metabolism to endogenous compounds
- abnormal seizure response to inducing agent
- abnormal serotonin level
- abnormal spatial learning
- abnormal surfactant secretion
- abnormal systemic arterial blood pressure
- abnormal systemic vascular resistance
- abnormal trabecular bone thickness
- abnormal type II pneumocyte morphology
- abnormal vascular branching morphogenesis
- abnormal vascular endothelial cell physiology
- abnormal vascular wound healing
- abnormal vasodilation
- absent alveolar lamellar bodies
- albuminuria
- anovulation
- bicuspid aortic valve
- cardiac hypertrophy
- cyanosis
- decreased adiponectin level
- decreased body temperature
- decreased body weight
- decreased bone mineral density
- decreased cardiac muscle contractility
- decreased circulating glucose level
- decreased circulating insulin level
- decreased coronary flow rate
- decreased corpora lutea number
- decreased fertilization frequency
- decreased heart rate
- decreased kidney cell proliferation
- decreased litter size
- decreased neurotransmitter release
- decreased ovulation rate
- decreased physiological sensitivity to xenobiotic
- decreased prostaglandin level
- decreased sensitivity to induced morbidity/mortality
- decreased survivor rate
- decreased vascular endothelial cell number
- decreased vascular permeability
- decreased vasodilation
- decreased white fat cell size
- delayed female fertility
- dilated heart left ventricle
- dilated renal glomerular capsule
- dilated renal tubules
- enhanced leukocyte tethering or rolling
- enlarged heart
- enlarged heart atrium
- expanded mesangial matrix
- fetal growth retardation
- glomerular capillary thrombosis
- glomerulosclerosis
- heart left ventricle hypertrophy
- hepatic steatosis
- hyperlipidemia
- hypertension
- hypoactivity
- impaired embryo implantation
- improved glucose tolerance
- increased anxiety-related response
- increased blood urea nitrogen level
- increased body weight
- increased bone mineral density
- increased brown adipose tissue amount
- increased cardiac muscle contractility
- increased circulating LDL cholesterol level
- increased circulating cholesterol level
- increased circulating creatine kinase level
- increased circulating estradiol level
- increased circulating free fatty acid level
- increased circulating glucose level
- increased circulating insulin level
- increased circulating leptin level
- increased circulating osteocalcin level
- increased circulating progesterone level
- increased circulating renin level
- increased circulating triglyceride level
- increased compact bone thickness
- increased fetal cardiomyocyte apoptosis
- increased heart left ventricle size
- increased heart rate
- increased heart right ventricle size
- increased heart ventricle size
- increased inflammatory response
- increased inguinal fat pad weight
- increased kidney apoptosis
- increased left ventricle systolic pressure
- increased liver weight
- increased mean systemic arterial blood pressure
- increased renal tubule apoptosis
- increased retroperitoneal fat pad weight
- increased susceptibility to bacterial infection
- increased susceptibility to bacterial infection induced morbidity/mortality
- increased susceptibility to induced colitis
- increased susceptibility to neuronal excitotoxicity
- increased susceptibility to weight gain
- increased susceptibility to weight loss
- increased systemic arterial blood pressure
- increased systemic arterial diastolic blood pressure
- increased systemic arterial systolic blood pressure
- increased vasoconstriction
- increased vasodilation
- increased ventricle muscle contractility
- increased white adipose tissue amount
- increased white fat cell size
- insulin resistance
- kidney degeneration
- kidney failure
- kidney inflammation
- kidney microaneurysm
- lung hemorrhage
- maternal effect
- mesangial cell hyperplasia
- mesangiolysis
- muscular ventricular septal defect
- neonatal lethality incomplete penetrance
- no abnormal phenotype detected
- ostium secundum atrial septal defect
- postnatal lethality incomplete penetrance
- premature death
- prolonged diestrus
- prolonged estrous cycle
- pulmonary alveolar edema
- pulmonary hypertension
- pulmonary vascular congestion
- reduced female fertility
- renal cast
- renal glomerulus fibrosis
- renal glomerulus hypertrophy
- renal glomerulus lipidosis
- renal hypoplasia
- renal interstitial fibrosis
- renal tubular necrosis
- respiratory distress
- short estrous cycle
- short femur
- short limbs
- small ovary
- syndactyly
- thick pulmonary interalveolar septum
- thin ventricular wall
- ventricular septal defect
role in cell
- phosphorylation in
- expression in
- production in
- chemotaxis
- cell death
- proliferation
- activation in
- migration
- contraction
- apoptosis
Subcellular Expression
Locations within the cell where the protein is known or predicted to be active, providing insight into its function and cellular context.
- Cytoplasm
- plasma membrane fraction
- caveolae fraction
- detergent-insoluble fractions
- membrane fraction
- endocytic vesicle membrane
- perinuclear Golgi regions
- cell surface
- Golgi region
- cell periphery
- intracellular space
- apical compartment
- perinuclear region
- intracellular membranes
- Nucleus
- Plasma Membrane
- Golgi Apparatus
- Mitochondria
- intercellular junctions
- cytoplasmic particles
- cytosol
- primary cilia
- cytoplasmic face of plasma membrane
- plasma membrane extracellular face
- caveolar membranes
- caveolae
- basolateral membrane
- Golgi membrane
- mitochondrial outer membrane
- neurites
- sarcolemma
- Z line
- membrane rafts
- filopodia
- perikaryon
- axons
- lamellipodia
- detergent-soluble fraction
Gene Ontology Annotations
Describes the biological processes, cellular components, and molecular functions associated with the mouse Nos3 gene, providing context for its role in the cell.
Biological Process
Functions and activities the gene product is involved in
- negative regulation of muscle hyperplasia
- positive regulation of guanylate cyclase activity
- negative regulation of cell proliferation
- vasodilation
- negative regulation of potassium ion transport
- endothelial cell migration
- regulation of the force of heart contraction by chemical signal
- positive regulation of Notch signaling pathway
- positive regulation of angiogenesis
- potassium ion transport
- endocardial cushion morphogenesis
- ovulation from ovarian follicle
- response to heat
- angiogenesis
- in utero embryonic development
- negative regulation of smooth muscle cell proliferation
- calcium ion transport
- negative regulation of calcium ion transport
- negative regulation of biomineral tissue development
- establishment of localization in cell
- response to fluid shear stress
- lung development
- aortic valve morphogenesis
- nitric oxide mediated signal transduction
- pulmonary valve morphogenesis
- negative regulation of blood pressure
- cell redox homeostasis
- regulation of systemic arterial blood pressure by endothelin
- response to hormone stimulus
- ventricular septum morphogenesis
- regulation of blood pressure
- mitochondrion organization
- positive regulation of blood vessel endothelial cell migration
- arginine catabolic process
- regulation of sodium ion transport
- blood vessel remodeling
- homeostasis of number of cells within a tissue
- smooth muscle hyperplasia
- removal of superoxide radicals
- positive regulation of gene expression
- negative regulation of platelet activation
- lipopolysaccharide-mediated signaling pathway
- response to lipopolysaccharide
- nitric oxide biosynthetic process
- regulation of neurological system process
Cellular Component
Where in the cell the gene product is active
- nucleus
- caveola
- cytoskeleton
- cytoplasm
- cytosol
- Golgi apparatus
- Golgi membrane
- endocytic vesicle membrane
- plasma membrane
Molecular Function
What the gene product does at the molecular level
- FMN binding
- heme binding
- actin monomer binding
- scaffold protein binding
- arginine binding
- calmodulin binding
- tetrahydrobiopterin binding
- NADP binding
- protein binding
- nitric-oxide synthase activity
- flavin adenine dinucleotide binding
- cadmium ion binding