dPCR Copy Number Variation (CNV) Probe Assay

用于通过单重或多重dPCR检测进行基因座特异性拷贝数变异及改变分析

200 多个经过湿实验验证的检测方案，以及用于计算机模拟实验设计的定制工具
染料可配置性支持在每个反应中对最多 5 个靶标进行多重检测
可提供目标基因检测、参比基因检测和着丝粒参比基因检测
已针对一系列样本（例如 FFPE、细胞系和 gDNA）进行了即用型检测验证
在 QIAcuity 仪器上实现简单快速的 dPCR 工作流程
可方便地使用 QIAcuity Software Suite 进行拷贝数数据分析

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CAT NO./ID 250101

QIAcuity Probe PCR Kit (1 ml)

1 ml 4x 浓缩 QIAcuity Probe Mastermix，2 x 1.9 ml 水

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CAT NO./ID 250102

QIAcuity Probe PCR Kit (5 ml)

5 x 1 ml 4x 浓缩 QIAcuity Probe Mastermix，8 x 1.9 ml 水

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CAT NO./ID 250103

QIAcuity Probe PCR Kit (25 ml)

5 x 5 ml 4x 浓缩 QIAcuity Probe Mastermix，4 x 20 ml 水

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CAT NO./ID 250111

QIAcuity EG PCR Kit (1 ml)

1 ml 3x 浓缩 QIAcuity EvaGreen 预混液，2 x 1.9 ml 水

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CAT NO./ID 69506

DNeasy Blood & Tissue Kit (250)

250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)

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CAT NO./ID 74106

RNeasy Mini Kit (250)

250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers.

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Resources

Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.

For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System

dPCR Copy Number Variation (CNV) Probe Assay

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