Assay name
HS_BRCA1_2474638 QuantiNova LNA PCR Assay
Wet-lab validated
qPCR wet-lab validated
Assay specification
Best coverage assay for BRCA1
Best transcript assay for ENST00000357654
Assay targets 13 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Best transcript assay for ENST00000357654
Assay targets 13 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Gene symbol
BRCA1
Gene aliases
RNF53;BRCC1;PPP1R53;FANCS
Species
Homo sapiens
Main target
ENST00000357654
Exons numbers
11-12
Ensembl exons ids
ENSE00003791246;ENSE00003527960
Amplicon start
4338
Amplicon length
110
Assay targets
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENST00000352993
Protein coding
Chr.17: 43044295 - 43125370
ENST00000357654
Protein coding
CCDS11453
NM_007294
Chr.17: 43044295 - 43125483
ENST00000461221
Nonsense mediated decay
Chr.17: 43045678 - 43125288
ENST00000461574
Protein coding
Chr.17: 43076537 - 43091824
ENST00000468300
Protein coding
CCDS11455
NM_007299
Chr.17: 43044805 - 43125451
ENST00000471181
Protein coding
CCDS11456
NM_007300
Chr.17: 43044295 - 43125483
ENST00000478531
Protein coding
Chr.17: 43063344 - 43125359
ENST00000484087
Protein coding
Chr.17: 43063344 - 43104916
ENST00000487825
Protein coding
Chr.17: 43076488 - 43104916
ENST00000491747
Protein coding
CCDS11454
NM_007298
Chr.17: 43045678 - 43125356
ENST00000493795
Protein coding
CCDS11459
NM_007297
Chr.17: 43045629 - 43125402
ENST00000493919
Protein coding
Chr.17: 43063360 - 43125402
ENST00000644379
Protein coding
Chr.17: 43045086 - 43091851