dPCR Mutation Assay WT1 21397 Human

Product Specification

AA Mutation: p.R458*
Nucleotide Mutation: c.1372C>T
dPCR wet-lab validated

Assay Name

dPCR Mutation Assay WT1 21397 Human

GeneGlobe Cat No (Assay ID)

DMH0000059

Species

Human (Homo sapiens)

Reaction size

200rxns and 1000rxns

Gene Symbol

WT1

Gene aliases

WAGR;WIT-2;AWT1;NPHS4;WT-1;GUD

Ensembl Gene ID

ENSG00000184937

Entrez Gene ID

7490

Genomic Mutation ID (COSV by COSMIC)

COSV60066326

Legacy Mutation ID (COSM by COSMIC)

COSM21397

Amino Acid Change

p.R458*

Nucleotide Change

c.1372C>T

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

GTCCTGGTGTGGGTCTTCAGGTGGTCGGACCGGGAGAACTTTCGCTGACAAGTTTTACACTGGAATGGTTTCACACCTAAATGGACAGAG

Amplicon length

Recommended Restriction Enzyme

CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX or ATTO550/ROX

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Introducing the dPCR Mutation Assay WT1 21397 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay WT1 21397 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay WT1 21397 Human and elevate your work to new heights.

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dPCR Mutation Assay WT1 21397 Human

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Product Specification

Resources

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