Assay name​
HS_POLB_2475143 QuantiNova LNA PCR Assay
Wet-lab validated​
qPCR and dPCR wet-lab validated
Assay specification​
Best coverage assay for POLB
Best transcript assay for ENST00000265421
Assay targets 9 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR and dPCR.
Assay in Focus Panel
Best transcript assay for ENST00000265421
Assay targets 9 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR and dPCR.
Assay in Focus Panel
Gene symbol​
POLB
Species​
Homo sapiens
Main target​
ENST00000265421
Exons numbers​
9-10
Ensembl exons ids​
ENSE00003673286;ENSE00003586441
Amplicon start​
637
Amplicon length​
76
Assay targets​
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENST00000265421
Protein coding
CCDS6129
NM_002690
Chr.8: 42338494 - 42371808
ENST00000518579
Protein coding
Chr.8: 42357174 - 42371752
ENST00000518925
Protein coding
Chr.8: 42338482 - 42369297
ENST00000519524
Retained intron
Chr.8: 42355459 - 42361712
ENST00000519771
Nonsense mediated decay
Chr.8: 42338605 - 42369329
ENST00000520008
Protein coding
Chr.8: 42338459 - 42361361
ENST00000521290
Protein coding
Chr.8: 42349039 - 42371808
ENST00000522610
Nonsense mediated decay
Chr.8: 42338507 - 42371765
ENST00000524208
Nonsense mediated decay
Chr.8: 42344960 - 42369988