Assay name
HS_SMAD4_2475230 QuantiNova LNA PCR Assay
Wet-lab validated
qPCR and dPCR wet-lab validated
Assay specification
Best coverage assay for SMAD4
Best transcript assay for ENST00000588745
Assay targets 5 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR and dPCR.
Assay in Focus Panel
Best transcript assay for ENST00000588745
Assay targets 5 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR and dPCR.
Assay in Focus Panel
Gene symbol
SMAD4
Gene aliases
DPC4;MADH4
Species
Homo sapiens
Main target
ENST00000588745
Exons numbers
7-8
Ensembl exons ids
ENSE00003654693;ENSE00003474913
Amplicon start
1143
Amplicon length
133
Assay targets
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENST00000342988
Protein coding
CCDS11950
NM_005359
Chr.18: 51030213 - 51085039
ENST00000398417
Protein coding
CCDS11950
Chr.18: 51029614 - 51085045
ENST00000588745
Protein coding
Chr.18: 51047047 - 51078467
ENST00000591126
Retained intron
Chr.18: 51052326 - 51079777
ENST00000592186
Nonsense mediated decay
Chr.18: 51047047 - 51078467