Assay name​
MM_NCF1_2088602 QuantiNova LNA PCR Assay
Wet-lab validated​
qPCR wet-lab validated
Assay specification​
Best coverage assay for Ncf1
Assay targets 6 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Assay targets 6 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Gene symbol​
Ncf1
Species​
Mus musculus
Main target​
ENSMUST00000126934
Exons numbers​
3-4
Ensembl exons ids​
ENSMUSE00001297338;ENSMUSE00000792565
Amplicon start​
573
Amplicon length​
80
Assay targets​
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENSMUST00000016094
Protein coding
CCDS71674
NM_001286037
Chr.5: 134220260 - 134229625
ENSMUST00000111275
Protein coding
CCDS39298
NM_010876
Chr.5: 134220260 - 134229625
ENSMUST00000126934
Retained intron
Chr.5: 134225717 - 134227810
ENSMUST00000139249
Retained intron
Chr.5: 134223445 - 134228399
ENSMUST00000144086
Protein coding
Chr.5: 134220260 - 134229625
ENSMUST00000146354
Nonsense mediated decay
Chr.5: 134220053 - 134229625