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dPCR LNA Mutation Assays

用于检测与癌症和肿瘤发生有关的特定 DNA 序列突变

  • 双重检测设计，可检测突变型及野生型序列
  • 可选 FAM + HEX 或 Atto 550 + ROX 荧光探针染料组合
  • LNA 加持的引物和探针，可提高检测的特异性和灵敏度
  • 经过湿实验验证的 dPCR 检测，有 200 多个靶标基因可供选择
  • 适用于循环肿瘤 DNA、液体活检、FFPE 和其他组织样本
  • 旨在搭配 QIAcuity Probe PCR Kit 一起使用

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Product List17 products

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GeneGlobe ID: DMH0000510 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 571 Human
AA Mutation: p.G13S
Nucleotide Mutation: c.37G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000336 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 561 Human
AA Mutation: p.G12R
Nucleotide Mutation: c.34G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000340 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 566 Human
AA Mutation: p.G12V
Nucleotide Mutation: c.35G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000188 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 563 Human
AA Mutation: p.G12S
Nucleotide Mutation: c.34G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000183 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 584 Human
AA Mutation: p.Q61R
Nucleotide Mutation: c.182A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000343 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 573 Human
AA Mutation: p.G13D
Nucleotide Mutation: c.38G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000339 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 565 Human
AA Mutation: p.G12A
Nucleotide Mutation: c.35G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000341 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 569 Human
AA Mutation: p.G13R
Nucleotide Mutation: c.37G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000344 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 574 Human
AA Mutation: p.G13V
Nucleotide Mutation: c.38G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000345 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 575 Human
AA Mutation: p.G13A
Nucleotide Mutation: c.38G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000349 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 585 Human
AA Mutation: p.Q61H
Nucleotide Mutation: c.183A>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000342 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 570 Human
AA Mutation: p.G13C
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000186 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 562 Human
AA Mutation: p.G12C
Nucleotide Mutation: c.34G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000347 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 581 Human
AA Mutation: p.Q61E
Nucleotide Mutation: c.181C>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000505 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 580 Human
AA Mutation: p.Q61K
Nucleotide Mutation: c.181C>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000180 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 586 Human
AA Mutation: p.Q61H
Nucleotide Mutation: c.183A>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000190 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay NRAS 583 Human
AA Mutation: p.Q61L
Nucleotide Mutation: c.182A>T
dPCR wet-lab validated
Product Specification
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Resources

学术海报 (2)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
用户使用手册 (1)
QIAcuity Application Guide
PDF (4MB)
产品介绍与指南 (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
快速启动实验方案 (1)
dPCR LNA Mutation Assays
PDF (175KB)
安全数据表 (1)
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
应用说明 (1)
Liquid biopsy-based detection of PIK3CA mutations from cfDNA using an end-to-end digital PCR workflow
PDF (2MB)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Safety Data Sheets (1)
Safety Data Sheets
Certificates of Analysis (1)
Certificates of Analysis
Brochures & Guides (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
Application Notes (1)
Liquid biopsy-based detection of PIK3CA mutations from cfDNA using an end-to-end digital PCR workflow
PDF (2MB)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Scientific Posters (2)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Quick-Start Protocols (1)
dPCR LNA Mutation Assays
PDF (175KB)
Instrument User Manuals (1)
QIAcuity Application Guide
PDF (4MB)
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