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GeneGlobe ID: DMH0000031 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay PIK3CA 754 Human

AA Mutation: p.N345K
Nucleotide Mutation: c.1035T>A
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay PIK3CA 754 Human
GeneGlobe Cat No (Assay ID)​
DMH0000031
Species​
Human (Homo sapiens)
Gene Symbol​
PIK3CA
Gene aliases​
PI3K
Ensembl Gene ID​
ENSG00000121879
Entrez Gene ID​
5290
Genomic Mutation ID (COSV by COSMIC)​
COSV55873276
Legacy Mutation ID (COSM by COSMIC)​
COSM754
Amino Acid Change​
p.N345K
Nucleotide Change​
c.1035T>A
Wildtype Allele​
T
Mutant Allele​
A
Mutation Strand​
+
Mutant description​
Substitution - Missense
Positive Control Sequence​
TGAATGGAGAAACATCTACAAAATCCCTTTGGGTTATAAATAGTGCACTCAGAATAAAAATTCTTTGTGCAACCTACGTGAATGTAAATATTCGAGACATTGATAAGGTAAAGTCAAATGCTGA
Amplicon length​
104
Recommended Restriction Enzyme​
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Product Resources

Validation Data
File Size: 88.1 KBLanguage: English

Resources

Scientific Posters (2)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Instrument User Manuals (1)
QIAcuity Application Guide
PDF (4MB)
Brochures & Guides (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
Quick-Start Protocols (1)
dPCR LNA Mutation Assays
PDF (175KB)
Safety Data Sheets (1)
Safety Data Sheets
Application Notes (1)
Liquid biopsy-based detection of PIK3CA mutations from cfDNA using an end-to-end digital PCR workflow
PDF (2MB)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Certificates of Analysis
Introducing the dPCR Mutation Assay PIK3CA 754 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay PIK3CA 754 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay PIK3CA 754 Human and elevate your work to new heights.
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