GeneGlobe ID: DMH0000254 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay EGFR 12422 Human

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Product Specification

AA Mutation: p.L747_A750delinsP
Nucleotide Mutation: c.2238_2248delinsGC
dPCR wet-lab validated
Assay Name​
dPCR Mutation Assay EGFR 12422 Human
GeneGlobe Cat No (Assay ID)​
DMH0000254
Species​
Human (Homo sapiens)
Reaction size​
200rxns and 1000rxns
Gene Symbol​
EGFR
Gene aliases​
ERBB1;ERRP;ERBB
Ensembl Gene ID​
ENSG00000146648
Entrez Gene ID​
1956
Genomic Mutation ID (COSV by COSMIC)​
COSV51782279
Legacy Mutation ID (COSM by COSMIC)​
COSM12422
Amino Acid Change​
p.L747_A750delinsP
Nucleotide Change​
c.2238_2248delinsGC
Wildtype Allele​
AATTAAGAGAAG
Mutant Allele​
AGC
Mutation Strand​
+
Mutant description​
Substitution - Missense
Positive Control Sequence​
CATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGT
Amplicon length​
106
Recommended Restriction Enzyme​
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Product Resources

icon_0245_cc_gen_pdf-s Validation Data
File Size: 124.94 KBLanguage: English

Resources

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Download Safety Data Sheets for QIAGEN product components.
应用说明 (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Safety Data Sheets (1)
Certificates of Analysis (1)
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