QIAseq UPX 3’ Transcriptome Kits

For high-throughput 3' transcriptome analysis from up to 10 ng of purified RNA or cell lysates using next-generation sequencing

Start with 10 pg to 10 ng of isolated total RNA, cell lysates
UPX tagging allows 4608–18,432 samples per single sequencing lane
UMIs eliminate library amplification bias for accurate gene expression
LNA-enhanced chemistry for increased accuracy, specificity and sensitivity
Includes access to the RNA-seq Analysis Portal for human, mouse and rat samples

Kits

For 3' gene expression analysis from cells or mRNA. Each kit contains cell lysis solution, buffers, oligos and enzymes lyophilized in a plate.

CAT NO./GG ID: 333088∕-

QIAseq UPX 3' Transcriptome Kit (96)

For 3' gene expression analysis from cells or mRNA of 8–96 samples at one time; each kit contains cell lysis solution, buffers, oligos and enzymes lyophilized in a 96-well plate which can be easily separated for processing 8–96 samples in parallel (this format is ideal for both small and large sample sizes)

INQUIRE

CAT NO./GG ID: 333089∕-

QIAseq UPX 3' Transcriptome Kit (96-M)

For 3' gene expression analysis from cells or mRNA of up to 384 samples; each kit contains cell lysis solution, buffers, oligos and enzymes in a 96-well plate. The increased volumes allow for automation or manual pipetting or where custom protocols are necessary.

INQUIRE

CAT NO./GG ID: 333090∕-

QIAseq UPX 3' Transcriptome Kit (384)

For 3' gene expression analysis from cells or mRNA of 384 samples simultaneously; each kit contains cell lysis solution, buffers, oligos and enzymes lyophilized on a 384-well plate for processing 384 samples in parallel (this format is best suited for large samples numbers of at least 384 samples)

INQUIRE

Indexes

For indexing on Illumina NGS instruments

CAT NO./GG ID: 333074∕-

QIAseq UPX 3' Trans. 12-Index (48)

For indexing up to 12 samples per sequencing lane on Illumina NGS instruments; multiple 96- or 384-well plates can be combined as one sample allowing for 12 x 96-well plates (1152 cells/wells) or 12 x 384-well plates (4608 cells/wells) per lane

ZAR 16,180.00

CAT NO./GG ID: 333075∕-

QIAseq UPX 3' Trans. 48-Index (192)

For indexing up to 48 samples per sequencing lane on Illumina NGS instruments; multiple 96- or 384-well plates can be combined as one sample allowing for 48 x 96-well plates (4608 cells/wells) or 48 x 384-well plates (18,432 cells/wells) per lane

ZAR 32,380.00

Resources

Part 1: Cell lysis and reverse transcription

Part 3: Adapter ligation and universal PCR

Part 2: Template amplification and fragmentation, end-repair, and A-addition