Assay Name
dPCR Mutation Assay PIK3CA 86044 Human
GeneGlobe Cat No (Assay ID)
DMH0000216
Species
Human (Homo sapiens)
Gene Symbol
PIK3CA
Gene aliases
PI3K
Ensembl Gene ID
ENSG00000121879
Entrez Gene ID
5290
Genomic Mutation ID (COSV by COSMIC)
COSV55881636
Legacy Mutation ID (COSM by COSMIC)
COSM86044
Amino Acid Change
p.E365K
Nucleotide Change
c.1093G>A
Wildtype Allele
G
Mutant Allele
A
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
TATATTTGAGTCTATCGAGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACCTACTTTTTTCTTTTAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAACACTCAAAGAGTACCTTGTTCCAATCCCAGGTAAGGAAGT
Amplicon length
154
Recommended Restriction Enzyme
AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC