dPCR LNA Mutation Assays

Para detectar mutaciones de secuencias de ADN específicas relacionadas con el cáncer y la oncogénesis

  • El diseño de ensayo doble permite detectar secuencias mutadas y nativas
  • Opción de combinación de colorantes fluorescentes FAM + HEX o Atto 550 + ROX
  • Los cebadores y las sondas mejorados con LNA aumentan la especificidad y la sensibilidad del ensayo
  • Ensayos de dPCR probados en laboratorios de química disponibles para más de 200 blancos
  • Ideal para su uso con ADN tumoral circulante, biopsia líquida, FFPE y otras muestras de tejido
  • Destinado a utilizarse con los QIAcuity Probe PCR Kits

Product

Product List4 products

GeneGlobe ID: DMH0000571 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ABL1 12576 Human
AA Mutation: p.Y272H
Nucleotide Mutation: c.814T>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000306 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ABL1 12560 Human
AA Mutation: p.T334I
Nucleotide Mutation: c.1001C>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000053 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ABL1 12574 Human
AA Mutation: p.E274V
Nucleotide Mutation: c.821A>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000573 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ABL1 12573 Human
AA Mutation: p.E274K
Nucleotide Mutation: c.820G>A
dPCR wet-lab validated
Product Specification
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Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)