dPCR LNA Mutation Assays

For detecting specific DNA sequence mutations related to cancer and oncogenesis

  • Duplex assay design detects mutated and wild-type sequences
  • Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
  • LNA-enhanced primers and probes increase assay specificity and sensitivity
  • Wet-lab tested dPCR assays available for more than 200 targets
  • Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
  • Intended for use with QIAcuity Probe PCR Kits

Product

Product List16 products

GeneGlobe ID: DMH0000204 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 746 Human
AA Mutation: p.R88Q
Nucleotide Mutation: c.263G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000034 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 773 Human
AA Mutation: p.M1043I
Nucleotide Mutation: c.3129G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000548 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 6475607 Human
AA Mutation: p.T1025=
Nucleotide Mutation: c.3075C>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000210 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 27505 Human
AA Mutation: p.K111N
Nucleotide Mutation: c.333G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000037 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 766 Human
AA Mutation: p.Q546K
Nucleotide Mutation: c.1636C>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000542 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 27502 Human
AA Mutation: p.E81K
Nucleotide Mutation: c.241G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000206 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 446019 Human
AA Mutation: p.E726K
Nucleotide Mutation: c.2176G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000031 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 754 Human
AA Mutation: p.N345K
Nucleotide Mutation: c.1035T>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000036 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 775 Human
AA Mutation: p.H1047R
Nucleotide Mutation: c.3140A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000209 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 774 Human
AA Mutation: p.H1047Y
Nucleotide Mutation: c.3139C>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000216 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 86044 Human
AA Mutation: p.E365K
Nucleotide Mutation: c.1093G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000292 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 763 Human
AA Mutation: p.E545K
Nucleotide Mutation: c.1633G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000033 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 764 Human
AA Mutation: p.E545G
Nucleotide Mutation: c.1634A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000050 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 12597 Human
AA Mutation: p.G1049R
Nucleotide Mutation: c.3145G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000062 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 776 Human
AA Mutation: p.H1047L
Nucleotide Mutation: c.3140A>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000212 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay PIK3CA 12459 Human
AA Mutation: p.Q546R
Nucleotide Mutation: c.1637A>G
dPCR wet-lab validated
Product Specification
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Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)