GeneGlobe ID: DCG0000155 | Cat. No.: 250210 | dPCR CNV Probe Assays

dPCR CNV Probe Gene of Interest for IRAK4

Gene symbol: IRAK4
Ensembl Gene ID: ENSG00000198001
dPCR wet-lab validated

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Product Specification

Product Name​
dPCR CNV Probe Gene of Interest for IRAK4
GeneGlobe Cat.No. (Assay ID)​
DCG0000155
Assay type​
Gene of Interest
Species​
Human (Homo sapiens)
Gene symbol​
IRAK4
Ensembl Gene ID​
ENSG00000198001
NCBI Gene Id​
51135
Strand​
Binds to forward (5’ to 3’) top genome strand
Amplicon length​
101
Amplicon region​
GTTTCTCAAGTGATGGAGATGACCTCTGCTTAGTATATGTTTACATGCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGTAAGCTATTTGTTCATCAGATTGTTTGGCTTTTTGTTTATATGCTGG
Recommended Reference Assay​
TERT (DCR0000186), AP3B1 (DCR0000238), RPP30 (DCR0000181), AGO1 (DCR0000536)
Recommended Restriction Enzyme​
CviQI, HaeIII, EcoRI, XbaI, PvuII
Wet-lab validated​
dPCR wet-lab validated
Hydrolysis Probe​
FAM, ATTO 550, Cy5
Primer Purification​
Desalted
Probe Purification​
HPLC

Resources

Application Notes (1)
Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.
Quick-Start Protocols (1)
Brochures & Guides (1)
For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System
Safety Data Sheets (1)
Certificates of Analysis (1)
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