dPCR LNA Mutation Assays

がんやがんの発生に関連した特異的なDNA配列の変異検出のために

デュプレックスアッセイ設計により、変異型と野生型の配列を検出
FAMとHEXまたはAtto 550とROXの蛍光色素の組み合わせを選択可能
LNAを組み込んだプライマーとプローブがアッセイの特異性と感度を向上
200以上のターゲットのdPCRアッセイをウェットラボで検証済み
循環腫瘍DNA、リキッドバイオプシー、FFPE、およびその他の組織サンプルでの使用に最適
QIAcuity MasterMixとの併用を想定

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Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.