dPCR LNA Mutation Assays

For detecting specific DNA sequence mutations related to cancer and oncogenesis

  • Duplex assay design detects mutated and wild-type sequences
  • Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
  • LNA-enhanced primers and probes increase assay specificity and sensitivity
  • Wet-lab tested dPCR assays available for more than 200 targets
  • Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
  • Intended for use with QIAcuity MasterMix

Product

Product List11 products

GeneGlobe ID: DMH0001102 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 9994994 Human
AA Mutation: p.S463C
Nucleotide Mutation: c.1388C>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001105 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 4774826 Human
AA Mutation: p.L536R
Nucleotide Mutation: c.1607T>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001109 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 1074639 Human
AA Mutation: p.Y537S
Nucleotide Mutation: c.1610A>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001104 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 6906109 Human
AA Mutation: p.L536P
Nucleotide Mutation: c.1607T>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001108 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 1074637 Human
AA Mutation: p.Y537C
Nucleotide Mutation: c.1610A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001101 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 4771561 Human
AA Mutation: p.S463P
Nucleotide Mutation: c.1387T>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001110 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 94250 Human
AA Mutation: p.D538G
Nucleotide Mutation: c.1613A>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001100 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 3829320 Human
AA Mutation: p.E380Q
Nucleotide Mutation: c.1138G>C
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001107 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 1074635 Human
AA Mutation: p.Y537N
Nucleotide Mutation: c.1609T>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001103 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 3621514 Human
AA Mutation: p.S463F
Nucleotide Mutation: c.1388C>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001106 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay ESR1 6843697 Human
AA Mutation: p.L536H
Nucleotide Mutation: c.1607T>A
dPCR wet-lab validated
Product Specification
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Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)