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Build confident multiplex dPCR panels - before you place your order

The dPCR Multiplex Assay Compatibility Checker on GeneGlobe screens your assay combination across five key parameters: amplicon length, amplicon overlap, fluorophore channels, heterodimer risk, and portfolio compatibility. Add up to 12 assays, catch conflicts instantly, and go into your experiment with confidence.

What is the dPCR Multiplex Assay Compatibility Checker?

The dPCR Multiplex Assay Compatibility Checker is available on GeneGlobe for all QIAGEN dPCR assay portfolios - CNV Probe Assays, LNA Mutation Assays, and Microbial DNA Detection Assays. Whether you are designing a respiratory pathogen panel, a liquid biopsy screen, or a custom research panel, the checker helps you identify and resolve assay conflicts before ordering.

The tool integrates seamlessly with the GeneGlobe assay catalog: search catalog products by target name, organism, or GeneGlobe ID, and include any custom assays you have already designed in GeneGlobe. Once your panel is validated, order directly from the results page or share a live link with a colleague for review - no GeneGlobe account required to view.

How to use the dPCR Multiplex Assay Compatibility Checker

Step-by-Step Summary

1. Add assays - Search by target name, organism, or GeneGlobe ID. Filter by assay type. Mix catalog and custom assays in the same check.
2. Run the check - Click Check compatibility. Results appear instantly across all five parameters.
3. Review results - See your overall rating and drill into any failing check with See details.
4. Refine your panel - Swap or remove conflicting assays, then re-run the check until your panel is ready.
5. Share or order - Download a PDF report, copy a shareable link, or add validated assays directly to your cart.

This tutorial walks through building a 6-assay respiratory pathogen panel, resolving a Weak Compatibility result caused by amplicon overlap and heterodimer errors, and achieving a clean 5/5 pass after panel redesign.

Common questions about dPCR Multiplex Assay Compatibility Checker

What assay portfolios are supported?

The checker supports QIAGEN's three dPCR assay portfolios: Microbial DNA Detection Assays, CNV Probe Assays, and LNA Mutation Assays. Custom assays designed in GeneGlobe can be included alongside catalog products.

How many assays can I check at once?

You can add up to 12 assays in a single compatibility check.

What parameters does the checker evaluate?

The checker screens five parameters: amplicon length, amplicon overlap, fluorophore channel compatibility, heterodimer risk, and portfolio compatibility.

What does the compatibility rating mean?

The tool returns an overall rating (Strong, Moderate, or Weak Compatibility) based on how many of the five checks pass. A rating of Moderate Compatibility (5/5 checks passing) is achievable even when a Portfolio warning is present - Portfolio warnings are informational and are not blockers.

What is a Portfolio warning?

A Portfolio warning appears when assays from different product lines are combined - for example, a CNV Probe Assay (such as RPP30 used as a reference gene) alongside Microbial Detection Assays. This is expected in many experimental designs and does not affect assay performance.

Can I include my own custom-designed assays?

Yes. Any custom assay you have designed using GeneGlobe's assay design tools will appear in the search panel and can be added to the compatibility check alongside catalog products.

Can I download a report?

Yes. Click PDF Download report from the results page to save a full compatibility report suitable for lab documentation or team review.

Does the checker specify instrument or kit requirements?

Yes. If your panel requires a specific QIAcuity kit version or instrument software version - for example, because one of your assays uses the ATTO700 fluorophore — the checker surfaces those requirements on the results page.

Where do I find the Multiplex Assay Compatibility Checker?

The checker is available on GeneGlobe under the dPCR assay portfolio pages. You can also access it from any dPCR assay product page or directly under Tools.

Can I share my results with a colleague?

Yes. Click Copy link to share to generate a link your colleague can open in any browser without a GeneGlobe account. They will see the full compatibility results and all check details.
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