GeneGlobe ID: DMH0000210 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay PIK3CA 27505 Human

AA Mutation: p.K111N
Nucleotide Mutation: c.333G>T
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay PIK3CA 27505 Human
GeneGlobe Cat No (Assay ID)​
DMH0000210
Species​
Human (Homo sapiens)
Gene Symbol​
PIK3CA
Gene aliases​
PI3K
Ensembl Gene ID​
ENSG00000121879
Entrez Gene ID​
5290
Genomic Mutation ID (COSV by COSMIC)​
COSV55876538
Legacy Mutation ID (COSM by COSMIC)​
COSM27505
Amino Acid Change​
p.K111N
Nucleotide Change​
c.333G>T
Wildtype Allele​
G
Mutant Allele​
T
Mutation Strand​
+
Mutant description​
Substitution - Missense
Positive Control Sequence​
CTTTTTAAAAGTAATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCAATCGAGAAATTGGTATGATACAATATCCTATTCTAAAATGCAAATAACCATAAAGCTTAACTGTTGTCCCTTTCTAAAAT
Amplicon length​
110
Recommended Restriction Enzyme​
CviQI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Product Resources

Validation Data
File Size: 87.16 KBLanguage: English

Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Introducing the dPCR Mutation Assay PIK3CA 27505 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay PIK3CA 27505 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay PIK3CA 27505 Human and elevate your work to new heights.